Can you please tell me syllabus for MSc biochemistry of entrance exam

Can you please tell me the syllabus for MSc biochemistry of entrance exam PGIMER?

Detailed Syllabus of M.Sc in Biochemistry Course

Physical Chemistry

Behaviour of Gases

Kinetic theory of gases
Absolute temperature scale
Ideal and real gas laws
Graham’s law of diffusion and its application to biological problems
Solution of Electrolytes
Pressure and volume concept
Equation of state
Dalton’s law of partial pressure
Non volatile and volatile electrolytes

Definition and type of solution
Colligative properties
Activity and activity coefficient
Elevation of boiling point
Laws of osmotic pressure
Determination of molecular weight
Osmotic behaviour of living cells
Expressing the concentration of solution
Roults’ law and molecular weight of the solute
Depression of freezing point
Vant Hoff theory of dilution
Theoretical explanation of osmosis
Turgor pressure
Acid, Bases and Buffers

Definition of pH
Effect of pH on protoplasmic components
Biological relevance of pH
pH indicators
Ionization of water
Exact treatment of the ionization of monoprotic acid in water
Arrhenius concept
Relation between initial acid concentration
Henderson-Hasselbalch equation
Titration of strong and weak acids with strong base
Salt hydrolysis
Exact treatment of the ionization of diprotic acid
Exact treatment of Bronsted lowery type monobase
Buffering range
Buffering capacity
Ist Law of Thermodynamics and Thermo chemistry

First law of thermodynamics
Relation between Cp and Cv in gaseous system
Energy changes in relation to changes in properties of the system
Enthalpy of a system
Change of internal energy and enthalpy in a chemical reaction
Determination of heats of reactions
Heat of formation, neutralisation, dilution and precipitations
Kirchoff’s law
Bomb Calorimeter and Dewar vacuum flask
Laws of Thermodynamics

Need and statement of the 2nd law
Concept of entropy
Helmholtz and Gibbs free energy functions
Maxwell’s relationships
Gibbs Helmoholtz equation
Gibbs-Duhem equation
Conditions for equilibrium and spontaneity
Law of mass action
Van’t Hoff-isotherm and law of chemical equilibrium
Dependence of equilibrium constant with temperature and pressure
Calapeyron-Clausius equation
Determination of fugacity and activity coefficient
Third law of thermodynamics
Variation of entropy with temperature
Determination of absolute entropies of liquids and gases
Applications of the III law of thermodynamics
Chemical Kinetics and Photochemistry

Reaction rate, order and molecularity
Collision and absolute theory of reaction rates
Factors influencing rates of reactions
Zero, I and II order reaction with examples
Mathematical treatments of rate constants of I and II order reactions
Calculation of activation energy
Organic Chemistry

Structure and Properties

Electronic configuration
Atomic and Molecular orbitals
Hybrid orbitals: sp, sp2 and sp3
Intermolecular forces
Covalent bond
Melting point
Boiling point
Acid and Base in organic chemistry
Alkanes and Radical reactions

Structure of methane, ethane, propane and butane
Production and reaction of radicals
Homolytic bond dissociation energies
Reactions of alkanes
Halogenation of methane
Halogenation of higher alkanes
Selectivity of bromine
Radical chain reactions
Alkyl halides and Ionic reactions

Structure, classification, nomenclature and physical properties of Alkyl halides
Reactions of Alkyl halides
Elimination reactions of Alkyl halides
SNI and SN2 reactions
Factors affecting rates of SNI and SN2 reactions

Reactions of Alkenes
Carbon-Carbon double bond
Structure of ethylene, propylene and butylenes
Substitution reactions and cleavage of Alkenes
Preparation of alcohols
Geometric isometric isomerism
Oxymercuration-demercuration and hydroboration oxidation processes

Structure and Nomenclature, Physical properties of Alcohols
Chemistry of OH group
Conversions of alcohols into mesylates, tosylates, alkyl halide and alkyl phosphates
Dehydration of alcohols
Intermolecular dehydration
Reactions of Alcohols
Synthesis of ethers from alcohols
Williamson synthesis
Methods of protecting –OH group
Oxidation of alcohols
Aldyehydes and Ketones

Structure, Nomenclature and Physical properties of Aldyehydes and Ketones
Nucleophilic addition to carbon-oxygen double bond
Reaction of Aldehydes and Ketones
Reduction to alcohols and hydrocarbons
Reductive emanation
Addition of water and alcohols
Additions of derivatives of ammonia
Addition of hydrogen cyanide and sodium bisulphite
Addition of Halides
Carboxylic acids and their Derivatives

Structure, Nomenclature, Physical properties and Acidity of carboxylic acids
Reactions of carboxylic acids
Substitution in alkyl or aryl group
Decarboxylation of carboxylic acids
Reactions of acyl chloride and acid anhydride
Reaction of Esters
Reduction to alcohols
Reactions with carbanion
Amines and Amides

Structure, Nomenclature, Physical properties and Basicity of Amines
Reactions of Amines with nitrous acid
Replacement and coupling reactions of Arenediazonium salts
Conversion to amides and sulfonamides
Hofmann and Cope eliminations
Reactions of Amides
Cell Biology

Basic Structure of Cell

Cell as a basic unit of living system
Biochemical composition of cell
Cell theory
Ultra structure of cell
Membrane composition
Early studies on Plasma Membrane
Membrane functions
Internal structure of cell and its functions

Structure and Function of the Endoplasmic reticulum
Golgi complex
Evolution of mitochondria and chloroplast
Photosynthetic process
Cell Nucleus

Structure and Function of Nucleus
Nucleosome and chromosome
Structure of Chromatin
Mitosis and Meiosis
Cell cycle
Nuclear envelope
Different processes in Cell

Cell-cell interaction
Cell locomotion
Cell senescence and death
Cell differentiation

Nucleic acids
Structure of DNA
DNA replication
Transcription and Post transcriptional modifications
Discovery of transformation and experiment of Hershey & Chase
Structure and types of RNA
Capping and splicing
Genetic Code

Evidence and Essentiality of Condon
Start and stop condones
Overlapping genes and reading frames
Universality of genetic code
Triplet code
Protein synthesis
Mechanism in prokaryotes and eukaryotes
Post translational modification and cell secretion

Mendelian Analysis, Chromosome Theory of Inheritance

Genotype and phenotype
Experiments of Mendel
Chromosome theory of inheritances
Work of TH Morgan
Work of Calvin Bridges
X-linked inheritance genetics and sexual cycle in haploids
Alternating haploids diploids
Linkage, mapping genes, interference, coincidence in pro- and eukaryotes
Extension of Mendelian Analysis

Variation of dominance relations
Lethal genes
Balance lethal system
Penetrance and expressivity
Segregation distortion
Gene interaction
Interaction between more than two gene pairs
Modifiers Linkage and Recombination
Chormosomes Structure and Mutations

Structural organisation of chromatin
Structure of Chromosome
Structural and numerical aberrations involving chromosomes
Spontaneous and induced mutations
Chemical and physical mutagens
Extrachromosomal Inheritance

Mitochondrial and chloroplast genetic system
Cytoplasmic Inheritance
Male sterility and maternal effects
Extra nuclear genomes
Population genetics
Microbial Geneticcs

Nature of Gene and Basic Genetic Engineering

One gene one enzyme hypothesis
Gene-protein relation
Manipulation of DNA
Restriction enzymes
Recombinant DNA
Vectors and cloning strategies
PCR technology

Concept of Biochemistry

Definition and scope of biochemistry
Units of length used in biochemistry
ATP-ADP cycle
Biomolecular Structure

Proteins and Amino Acids
Nucleic Acids

Nature of enzyme catalysis
Naming of enzymes
Michaelis-Menten concept of enzyme action
Active binding and catalytic sites
Factors affecting enzymes catalyzed reactions
Control of enzyme activity
Multi enzyme systems
Metabolic Generation of Energy
Energy relationship between catabolic and anabolic pathways
Stages in the extraction of energy from food stuff
Generalized concept of glycolysis
Kreb’s cycle
Glycogen synthesis
Oxidative phosphorylation and electron transport chain
Utilization of Energy

Energy utilization in anabolic processes
Dark reaction of photosynthesis
Biosynthesis for storage